Friday, 9 May 2014

CELL:THE FUNDAMENTAL UNIT OF LIFE

Chapter – 6
The Fundamental unit of life – Cell
Q) What is cell?
Cell is the smallest structural and functional unit of life.
Q) Who discovered cell?
Robert Hooke in the year 1665. He observed a thin slice of cork under his microscope and observed small hexagon boxes like that in a honey comb and he named this boxes as cells. He discovered dead and fixed cells.
Q) Who discovered the free living cells for the 1st time?
Anton Von Leeuwenhoek, discovered the free living cell in the pond water for the 1st time in the year 1674.
Q) Who discovered the nucleus in the cell ?
Robert Brown in the year 1831.
Q) Who coined the term “protoplasm”?
Purkinje in the year 1839.
Q) What is “cell theory”?
According to the cell theory, all the plants and animals are composed of cell , and the cell is the basic unit of life. It was proposed by two biologist – 1) Matthias Jacob Schleiden (1838)      2) Theodore Schwann (1839)
It was further expanded by Rudolf Virchow (1855) by suggesting that all cells arrives from the “pre-existing cells”.
Q) What made the observations of the ultra structure of the cell possible?
The discovery of the electron microscope in 1940 made it possible to observe and understand the complex structure of the cells and its various organelles (on the basis of the no. of cells how organisms are classified?)
Two parts – 1) Unicellular organisms – Amoeba, Paramecium.
                      2) Multicellular organism – Cells group together in a single body and assume different
                          functions in it to form various body.
Q) Why the different cells present in the body of an organism looks different?
The different parts of the human body has different functions, Since They require the cells of different type in which each type of cell organelles perform specific functions, Since the different organ system are made up of different types of cells.



Q) Differentiate between unicellular and multicellular organism?
S.No.
Unicellular Organism
S.No.
Multicellular Organism
1.
2.
3.
4.


5.
Made up of single cell .
Simple organization.
They are primitive, came first on earth.
Singel cell perform all the activities, so there is no division of labour.

Cell division always leads to reproduction.
1.
2.
3.
4.


5.
Made up of many cells.
Complex organization.
They are advanced, came later on earth.
Different Functions are performed by different cells, tissues, organs, Since there is proper division of labour is seen.
May or may not be related to reproduction.

Q) Differentiate between Prokaryotic and Eukaryotic cell.
S.No.
Prokaryotic Cell (Organism)
S.No.
Eukaryotic Cell (Organism)
1.

2.

3.
4.

5.
6.
Pro – primitive/first , karyon – nucleus. (false)
True nucleus is absent rather nucleus like structure called nucleoid is present.
They originated first on earth.
They don’t have membrane bound cell organelles.
Eg.-  Blue green Algae
The Cell division is very simple, mitosis and miosis, are absent.
Eg.- Eubacteria, Archae- bacteria.
1.

2.

3.
4.

5.
6.
Eu- New / true, karyyon – nuleus

True nucleus is present.

They originated later on earth.
They have membrane bound cell organelles.

Eg.- Mitochondria.
Mitosis and Meiosis are present.
Eg.- Protists and animals.

 Q) What do you mean by organelle?
A specific living structure that performs a specific jobs for cells and helps it to survive is called an organelle.
Q) What are chromosomes?
They are short, rod  like structure  formed inside the nucles at the time of cell division from the chromatin threads.
Q) What are genes?
Genes are small functional segment of DNA that codes for character.




























Q) How does a cell performs various activities for its survival?
There is an efficient division of labor found inside the cells. The cells have tiny components inside it called organelles, (miny organs) These organelles have a specific structure and each one of them is specialized in  performing specific vitae activity such as making of new.
Q) Plasma Membrane!
It is a very thin, eleastic, dynamic, semi-fluid membrane made up of lipo-protien that separete the cell from external environment and gives identity to the cell. It is present in all types of cell.
Q) Why plasma-membrane is called selectively permeable?
The plasma membrane allows & permits the entry of some material in and out of the cell. It also prevents the movement of some other material out of the cell, Since, it is called a selectively permeable membrane.
Q) What is Active & Passive transport?
The plasma membrane allows 2 types of transport, they are as follows:-
S.No.
Active
S.No.
Passive
1.

2.

3.
It is a specialized process that needs energy
Substance move from lower to higher concentration
The substance moves against the concentration gradient
1.

2.

3.
It is the normal process that does not allow the consumption of energy
Substance move from heigher to lower concentration
The substance moves along the concentration gradient

Q) Define “cell wall ”.

       It is made up of cellulose which is formed of very thin fibres of glucose along with “pectin”
       Cellulose is a complex substance and provide structural strength in the plant.
       It is strong and rigid
       It is present in plant cell only
       It resist bursting due to entry of excess water into the cell
       In fungai, cell wall is made up of “chitine”. And in bacteria, it is made up of “peptidoglycan
       There are large empty spaces present between the cellulose fibres, therefore it is freely permeable.
Q) What is plasmolysis?
When a living plant cell looses water through osmosis, there is a shrinkage or contraction of the content of the cell away from the cell wall, this phenomenon is known as “plasmolysis”
However, only living cell and not the dead cells are able to absorb slash, loose water by osmosis so are able to do plasmolysis.
Q) Define “cell membrane”
       Allows both active and passive movement of molecules
       Allows the diffusion of oxygen and carbon dioxide
       A living, semi fluid and dynamic layer
       It can control the movement of materials IN and OUT of the cell.
Q) What is diffusion ?
Diffusion is a spontaneous movement of substance from a region of high concentration to a region of lower concentration.
It is a property of particles of solid, liquid and gaseous particles due to their kinetic energy. diffusion plays an important role in gaseous exchange. i.e. movement of carbon dioxide and oxygen across the cell membrane between the cell as well as by its external environment
Q) How water moves in and out of the cell ?
The movement of water molecules in plasma membrane is a special type of diffusion called osmosis.
Osmosis is a passage of water from a region of high water concentration through a semi-permeable membrane to a region of lower concentration however osmosis is affected by the amount of substance dissolved ( osmosis is potential in water).
OSMOSIS IS OF TWO  TYPES :--
       EX-OSMOSIS – loss of water by a cell due to the shrinkage ( or which it shrinks)
       END-OSMOSIS— gain of water by a cell due to which it swells
Unicellular fresh water organisms and most of the plant cell tend to gain water through osmosis. Absorption of water by plant root is also an example of osmosis.
THREE TYPES OF SOLUTION WITH RESPECT TO CONCENTRATION :--
       Hypo-tonic solution — if the medium surrounding the cell has a higher water concentration then the cell, meaning that the outside solution is very dilute then such a solution is known as hypo-tonic solution.   
 A cell will gain water by osmosis, when placed in such a solution
Water molecules are free to pass across the cell membrane in both the directions but more water would come into the cell than will leave. The net result would be that due to more  water enters the cell, the cell is likely to swell up.
       Hyper- tonic solution — if the surrounding medium has a lower concentration. Meaning that it has a very concentrated solution (having more solute) such a solution is known as hyper-tonic solution.
A cell will lose water by osmosis, when placed in such a solution.
Water crosses the cell membrane in both direction but this time less water will leave the cell than enters, therefore the cell will shrink
       Iso-tonic — if the surrounding has exactly the same water concentration as the cell is having, then such a solution is known as a Iso-tonic solution
There will be no net gain or loss of water across the cell membrane, when the cell is placed in such a solution
Water crosses the cell membrane in both the direction but the amount of water going in is same as the amount coming out. So there is no movement of water, therefore the cell size will remain the same.
CYTOPLASM.
       It is a jelly like structure enclosed by the cell membrane, it is also known as the cell matrix
       It provides a medium for bio-chemical reaction to take place. It is made up of water and many organic and inorganic molecules dissolved and suspended in it.
       It provides platform for all the organelles ( living and non-living). Eg chloroplast, mitochondria, waste particles  etc
           

NUCLEUS
       It controls the normal metabolic activities of the cell, this is why it is also known as the brain of the cell.
       It is bounded by two nuclear membrane, both forming a nuclear envelope with lots of small pores for exchange of materials with the cytoplasm.
       It contains the genetic material, i.e. “DNA” in the form of thread like structure called chrmatins.

What is membrane bio-genesis?
Some of the proteins and lipid help in bulilding the cell membrane, which is synthesid by ER, this process is known as bio-genesis.
Cell Organelles
       The tiny structure present inside the cell is called cell organelles
       they all suspended in the cytoplasm
       Usually they are membrane bound.
       Exception- Ribosomes & Centrioles.
       they have specific organizqaton and performs one or more vital functions.
       Due to this there is division of labour inside the Eukaryotic cell.

ENDOPLASMIC RETICULUM
       It is a network of scattered inter-connected membrane bound tubes/chanels & vesicles that mainly helps in the movement of various molecules. It consists of three main components- 1) CISTERNE (hollow sac like structure)         2) TUBULES (long & hollow)     3) VESICLES (pouch like)
       It increases the inner surface area for efficient camical activity.
       It helps in trafficing of various molecules.
S.No.
Rough ER
S.No.
Smooth ER
1.
2.

3.

More scattered.
It appears rough because of the presence of Ribosomes on its surface.
Mainly associated with protein synthesis.
1.
2.

3.
Less scattered.
It appears smooth because Ribosomes are absent.
Mainly associated with lipid synthesis.


Function or ER:-
1.                 It serves as channels for the transport of materials between various region of th cytoplasm or between the cytoplasm & the nucleus.
2.                 It functions as a cytoplasm framework providing a surface for some of the bio- chemical activities of the cell
3.                 In th live cells of the vegetables, SER plays a crucial role in dedtoxifying many poisons & drugs.
4.                 It provides internal support to the cell and helps to maintain the shape opf the cell.

GOLGI BODIES
                   It is discovered by famous italian scientist Camillo Golgi.
                   It is made up of sac like cisterns lying almost parallel one above the another, with two faces forming an maturation which are convex & concave respectively.
                   Golgi is formed by the transmition vesicles from ER containing mixed products.
                   From the maturation face, sorted products are packed into vesicles , and sent to different targets.
                   The poorly developed golgi in plants cell is better known as "Dictyosomes".
Functions:-
1.                 It packs and depatches materials from ER to various targets.
2.                 It is associated with storage, modification & packaging of products.
3.                 Their the complex sugar are made from simple sugar.
4.                 Formation of lysosomes takes place in Golgi bodies.

LYSOSOMES
                   These are a kind of waste disposal system for cells that helps in the process of digestion, and removal of debtis.
                   these are oval or bound pouch like vesicles containing around 40 hydrolytic, digestive enzymes.
Q) How lysosomes helps in process of cleaning & digestion?
Lysosomes helps to clean the cell by digesting any foreign material as wll as wornout cell organelles. Foreign material entering the cell, such as bacteria or food as well as old organelles end up in the lysosomes which break them up into small pieces with the help of digestive enzymes.
Q) Why lysosomes are called "Suicidal Bags"?
Tjhe powerful digestive enzymes present inside the lysosomes are capable of breaking down all the organic materials due to te disturbance in the cellular matabolism eg.- When the cell gets damaged, lysososmes may burst and the enzymes digest there own cell. "Therefore lysosomes are also known as Suicidal bags".

MITOCHONDRIA
These are elongated, large, rod shaped double membraneous organelles which is a center of cellular respiration.
                   The outer membrane is smooth & porous while the inner membrane has got a no. of indolding called "cristae" which increase the internal surface area for efficient metabolic reaction.
                   The inner membrane walls match-stick like particles called "oxysosomes" for "ATP synthesis"
                   The jelly like material filled inside is called "Matrix" that contain enzyme for respiration.
                   Mitochondria ahs its own "DNA and Ribosomes" and are able to produce some of the required proteins & enzymes. It is "Semi- Autonomou Organelles".


Functions:-
1.                 It is a place of aelobic respiration where food is oxydised to release energy.
2.                 It is a place of ATP synthesis.
3.                 Many entermidiate compounds are synthesised inside the mitochondria which are used for different cemical activities.

PLASTIDS
                   Its is double membrane bound coloured organell found only in plant cell (and some microbe)
                   It is second largenst organell in the plant cell
                   Plastids are of 3 types
1.                 Chloroplast- Site of photosynthesis. It is known as the "kitchen of the cell".
2.                 Chromoplast- Providesa colour to the different plants part. It helps in pollination.
3.                 Leucoplast- Envolves in foor storage. It stores starch, oils, protiens granulles.

                   Plastids are semi-autonomous because they have their own DNA and Ribosomes so they can also synthesize some of their protiens.


RIBOSOMES
                   It is the smalles cell organells.
                   It is made up of 2 units and is chemically decomposed of RNA & protein.
                   It is smembrane less.
                   It is called the "protien factory of the cell", because it synthesis various types of protiens for varius types of protein (cellular activities)

VACUOLES
                   These are membrabe - bound pouch like structure filled with some fluid. In planty cell there is a large vacuole in the middle of each cell containing food materials.
                   In an animal cell there are many small vacuoles scattered in the cytoplasm.
                   The vacuole are full of cell cap and provide tergidity & rigedity to the cell.
                   Many important substance are stored in the vacuole which include amino acids, sugar, various organic acid and protein.

CENTROSOMES
       It is cell organell found in animal cell and cnsdist of two hollow rod like structure called centrioles. Surrounded by a clear area.
       Centrioles are made up of protein and are located bear the nucleues.
       It helps in the process of cell division .
       It sometime give rise to flagella.

Q) Mention the role of vacuole in uni-cellular organism?d
In uni-cellulare organism, like amoeba the food vacuole contains food items that amoeba has consume. In such organism, specialised vacuole also play an imortant role in expelling exess water and some waste from the cell.
Q) How can we see the different parts of a cell when the cell is almost transparent?
We can make different parts of cell visible by using specific colours called stains. These are special colouring agent which are differently absorbed by different cell organism (Organell) and produce proper contact due to which the parts become prominently visible,some name of common stains are:-
       Rosin - Cytoplasm & The cell wall
       Safeanine - Cytoplasm & The cell wall
       Haemotonylin - Nucleus
       Methylene Blue – Microbes
S.No.
Plant Cell
S.No.
Animal Cell
1.
2.


3.
4.

5.
They are usually regular in shape.
It has an outer covering that is cell wall made up of cellulose in addition to the cell membrane.
Plastids are present.
Vacuole are less in no. and bigger in size.

Centrioles are absent
1.
2.


3.
4.

5.
They are usually irregular in shape.
Cell wall is absent


Plastids are absent.
Vacuole are more in no. and are smaller in size.
Centrioles are present near to the nucleus.








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